How To Calculate Rf Values Of Pigments

The rf value for aspirin is 125148 equals 084.

How to calculate rf values of pigments. How to calculate rf values for tlc calculation of rf value. For ibuprofen the rf value is 132148 equals 089 and for acetaminophen the rf value is 107148 equals 072. Rf value distance travelled by the pigment distance travelled by the solvent r f value table for the solvent used 90 propanol 10 ether pigment r f value carotene 094 xanthophyll 089 chlorophyll a 046 chlorophyll b 022. R f values can be used to identify unknown chemicals if they can be compared to a range of reference substances.

Rf stands for retardation. Measure the distances travelled by the solvent and the pigments and calculate the retardation factor rf using the following equation. Tlc is a chromatography technique responsible for the separation of organic compounds on a mixture based on their relative mobility. The rf value is defined as the ratio of the distance moved by the solute ie.

Rf distance travelled by pigment distance travelled by solvent. The rf value for a particular substance is always the same if the same solvent and. To do this you measure the r f values. Mobile phase mobility rf value stationary phase separation thin layer chromatography tlc what is tlc.

One component called component 1 moved 22 cm and the other moved 39 cm called component 2. The solvent moved 45 cm. To calculate the rf value for each pigment use the following formula. The dye or pigment under test and the distance moved by the the solvent known as the solvent front along the paper where both distances are measured from the common origin or application baseline that is the point where the sample is initially spotted on the paper.

To see which standard these components correspond to you will need to use the retention factor formula to calculate the r f values.